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KMID : 1161420190220060623
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Journal of Medicinal Food
2019 Volume.22 No. 6 p.623 ~ p.630
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Artemisia princeps Inhibits Growth, Biofilm Formation, and Virulence Factor Expression of Streptococcus mutans
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Yang Yang
Hwang Eun-Hee
Park Bog-Im
Choi Na-Young
Kim Kang-Ju
You Yong-Ouk
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Abstract
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This study was designed to determine whether the ethanol extract of Artemisia princeps could inhibit the cariogenic activity of Streptococcus mutans. The increase in acid production and biofilm formation by S. mutans were evaluated. The expression levels of virulence factor genes were determined by performing the real-time polymerase chain reaction (PCR). The bactericidal effect was tested by confocal laser scanning microscopy. The A. princeps extract was observed to inhibit the growth of S. mutans at concentrations >0.05?mg/mL (P?0.05?mg/mL. These experimental results were similar to that observed with the scanning electron microscopy. The results of the confocal microscopy revealed that the A. princeps extract at high concentrations of 0.4?3.2?mg/mL showed a bactericidal effect in a concentration-dependent manner. According to the results of the real-time PCR analysis, it was observed that the A. princeps extract inhibited the expression of virulence factor genes. These results suggest that A. princeps may inhibit the cariogenic activity of S. mutans, and may be useful as an anticariogenic agent.
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KEYWORD
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antibacteria, Artemisia princeps, dental biofilm, gene expression, Streptococcus mutans, virulence factor
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